| | 主な研究成果 |  |
| (1)論文発表 (国内0件、海外16件) | |
| 1) Tabara, H., Motohashi, T. and Kohara, Y.: A multi-well version of in situ hybridization on whole mount embryos of C. elegans. Nucl. Acids Res. 24, 2119-2124 (1996). | |
| 2) Kuwahara, M., Ishibashi, K., Gu, Y., Terada, Y., Kohara, Y., Marumo, F. and Sasaki, S.: A water channel of the nematode Caenorhabditis elegans and its implications for channel selectivity of MIP proteins. The Am. J. Physiol.: Cell Physiol. C1459-C1464 (1998). | |
| 3) The C. elegans Sequencing Consortium: Genome sequence of the nematode C. elegans: A platform for investigating biology. Science 282, 2012-2018 (1998). | |
| 4) Tabara, H., Hill, R.J., Mello, C., Priess, J. and Kohara, Y.: Pos-1 encodes a cytoplasmic zinc-finger protein essential for germline specification in C. elegans. Development 126, 1-11 (1999). | |
| 5) Fields, S., Kohara, Y. and Lockhart, D.J.: Functional Genomics. Proc. Natl. Acad. Sci. USA 96, 8825-8826 (1999). | |
| 6) Motii, M., Yoshida, S., Morita, K., Kohara, Y. and Ueno, N.: Identification of TGF-b-regulated genes in Caenorhabditis elegans by differential hybridization of arryaed cDNAs. Proc. Natl. Acad. Sci. USA, 96, 15020-15025 (1999). | |
| 7)Cassata, C., Kagoshima, H., Andachi, Y., Kohara, Y., Durenberger, M.B., Hall, D.H., and Burglin, T.R.: The Lim Homeobox Gene ceh-14 Confers Thermosensory Function to the AFD Neurons in Caenorhabditis elegans. Neuron 25, 587-597 (2000) | |
| 8) Asahiha, M., Ishihara, T., Jindra, M., Kohara, Y., Katsura, I. and Hirose, S.: A member of the ancient nuclear receptor class Ftz-F1 is required for embryogenesis, molting and reproduction in Caenorhabditis elegans. Genes to Cells 5, 711-723 (2000). | |
| 9) Nathalie Pujol, Claire Bonnerot, Jonathan Ewbank, Yuji Kohara and Danielle Thierry-Mieg: The C. elegans unc-32 gene encodes alternative forms of the 100-kDa a subunit of Vacuolar-ATPases. J. Biol. Chem. (2001 in press) | |
| 10) Maeda, I., Kohara, Y., Yamamoto, M., and Sugimoto, A. (2001). Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Current Biology, 11 No.3, 171-176. | |
| 11) Reboul, J., Vaglio, P., Tzellas, N., Thierry-Mieg, N., Moore, T., Jackson, C., Shin-I, T., Kohara, Y., Thierry-Mieg, D., Thierry-Mieg, J., Lee, H., Hitti, J., Doucette-Stamm, L, Hartley, J., Temple, G., Brasch, M., Vandenhaute, J., Lamesch,Pl., Hill, D. & Vidal, M.: Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C.elegans. Nature Genetics, 27, 332-336 (2001) | |
| 12) Rosa E. Navarro, Eun Yong Shim, Yuji Kohara, Andrew Singson and T. Keith Blackwell: cgh-1, a Conserved Predicted RNA Helicase Required for Gametogenesis and Protection from Physiological Germline Apoptosis in C. elegans. Development (in press) | |
| 13) Momoyo Hanazawa, Makoto Motii, Naoto Ueno, Yuji Kohara and Yuichi Iino: Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA, 98, 8686-8691 (2001). | |
| 14) Zeynep Altun-Gultekin, Yoshiki Andachi, Ephraim L. Tsalik, David Pilgrim, Yuji Kohara and Oliver Hobert: A regulatory cascade of three homeobox genes, ceh-10, ttx-3 and ceh-23 , controls cell fate specification of a defined interneuron class in C. elegans. Development 128, 1951-1969 (2001) | |
| 15) Ken-ichi Ogura1,3, Shohei Mitani2, Keiko Gengyo-Ando2 and Yuji Kohara1: Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein PIP-1 in Caenorhabditis elegans. (Submitted) | |
| 16) Anahita Amiri, Brett D. Keiper, Ichiro Kawasaki, Yuan Fan, Yuji Kohara, Robert E. Rhoads and Susan Strome: An isoform of elF4E is a component of germ granules and is required for spermatogenesis in C. elegans. Development 128, 3899-3912 (2001) | |
| (2)口頭発表(内容が重複しているものは除く。国際学会発表を優先。) | |
| 1) 招待、口頭講演 (国内27件、海外17件) | |
| Kohara, Y.: Expression map of the C.elegans genome. The Second France-Japan Bilateral Genome Workshop, Paris, France, March (1996). | |
| Kohara, Y.: International Symposium “Network and Evolution of Molecular Information“, Tokyo, April (1996). | |
| Kohara, Y., Motohashi, T., Tabara, H., Watanabe, H., Sano, M., Miyata, A., Mitani, Y., Ohba, T., Iida, K. & Uesugi, H.: Expression pattern map of the C. elegans genome. HGM’97 Tronto, Canada, March (1997) | |
| Kohara, Y.: Expression pattern map of the C.elegans genome. HUGO Pacific Meeting, Pusan, Korea, August (1997) | |
| Kohara, Y.: Embryonic specification of germ line in C.elegans. International Symposium “Gene Functions and Cell Differentiation“, Mishima, September (1997) | |
| Yuji Kohara, Tomoko Motohashi, Hiroaki Tabara, Hisako Watanabe, Masako Sano, Akiko Miyata, Yuko Mitani, Tokie Ohba, Kouitchi Iida, Hiroko Uesugi, Keiko Hirono, Tadasu Shin-I, Masumi Obara: Expression Pattern Map of the C,elegans Genome 11th International C.elegans Meeting, May28∼June3 1997, Madison,WI, USA. | |
| Yuji Kohara: Expression Pattern map of the C.elegans Genome: 7th International workshop on the Identification of Transcribed Sequences November16-18 1997, Montrey, CA, USA | |
| Kohara.Y,: The Genome as the Blueprint of Life. 1st Annual Symposium on Japanese-American Frontiers of Science, Aug.21-23, 1998 Irvine, CA, USA | |
| Kohara.Y,: Post genomics strategies to understand the molecular mechanisms of C.elegans development. 2nd HUGO Paciffic Meeting, 24-26 March 1999, Bali Indonesia. | |
| Kohara.Y,: Post genomics strategies to understand the molecular mechanisms of C.elegans development. Human Genome Meeting 1999, 27-30 March Brisbane, Australia | |
| Yuji Kohara, the Kohara Lab and collaborators: Post genomics strategies in C.elegans. 12th International C.elegans Meeting, June2-6,1999, Madison, WI, USA | |
| Ogura, K. and Kohara, Y.: Studies on POS-1 interacting proteins. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Kohara, Y.: Post-genomics strategies in C.elegans toward understanding of the molecular mechanisms of development. 6th IUBMB Conference, Oct. 10-13, 1999, Seoul, Korea. | |
| Kohara, Y.: POST-GENOMICS STRATEGIES IN C.ELEGANS TOWARD UNDERSTANDING OF THE MOLECULAR MECHANISMS OF DEVELOPMENT. 9th Beyond the Identification of Transcribed Sequences Workshop, Oct. 28-31, 1999, Washington DC, USA. | |
| Kohara, Y., Itoh, M., Motohashi, T., Minakuchi, Y., Shin-i, T., Onami, S., Nagaoka, T., Ogura, K. and Hirono, K.: A 4-dimentional database of gene expression and function in C.elegans-Towards “C.elegans as a test tube in silico“ | |
| The 2nd International Workshop on Advanced Genomics “Genomics and Drug Discovery“, 27-28 April, 1999, Chiba, Japan | |
| Kohara, Y.: Genome Biology of C.elegans Development. 3rd HUGO Pacific Meeting, Oct. 18-21, 2000, Shanghai. | |
| Kohara, Y.: Gene Expression Patterns in Caenorhabditis elegans. The 8th International Conference: Peace through Mind/Brain Science, Feb. 3, 2000, Hamamatsu. | |
| Kohara, Y.: Genome Biology of C.elegans Development. The 14th International Mouse Genome Conference, Nov. 8, 2000, Narita. | |
| Kohara, Y.: Comprehensive measurement of C.elegans gene expression. The 1st International Conference on Systems Biology, Nov. 16, 2000, Tokyo. | |
| Kohara, Y.: Functional genomics of C.elegans development. 14th International Congress of Developmental Biology, July 9, 2001, Kyoto. | |
| Yuji Kohara, Ikuko Sugiura, Tokie Oba, Masumi Obara, Takami Suzuki, Tadasu Shin-i, Yumiko Ueta, Mina Iwata, Keiko Hirono, Kyoko Nakata, Masahiro Ito, Yohei Minakuchi, Michiko Serizawa: Developmental expression map of C.elegans genome. 13th International C.elegans Meeting, June 22-26 (2001), Los Angeles, USA. | |
| Danielle et Jean Thierry-Mieg·Yutaka Suzuki, Sumio Sugano, Kazuko Oishi, Masako Sano, Hisayo Nomoto, Shinobu Haga, Satoko Nishizaka, Hiroko Hayashi, Fumiko Ohta, Sachiko Miura, Hiroko Uesugi, Yann Thierry-Mieg, Vahan Simonyan, Adam Lowe, Tadasu Shin-I and Yuji Kohara: The worm transcriptome project. 13th International C. elegans Meeting, June 22-26 (2001), Los Angeles, USA. | |
| Ken-ichi Ogura, Shohei Mitani, Keiko Gengyo-Ando and Yuji Kohara: Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein PIP-1. 13th International C. elegans Meeting, June 22-26 (2001), Los Angeles, USA. | |
| Robert Barstead, Anil Dsouza, Mark Edgley, Keiko Gengyo-Ando, Erin Gilchrist, Jeff Holmes, Steven Jones, Yuji Kohara, Martin Lansdale, Sheldon McKay, Etsuko Machiyama, Shohei Mitani, Don Moerman, Gary Moulder, Sachiko Noguchi, Jamie Osborn, Bin Shen1, Miwa Tamura, Malini Viswanathan: The C. elegans Knockout Consortium: Interim Report. 13th International C.elegans Meeting, June 22-26 (2001), Los Angeles, USA | |
小原雄治: 「生物学者から見た分子生物情報学: 生命プログラムと2000年問題」
第7回分子生物情報(SIGMBI)研究会(人工知能学会第2種研究会)1999年5月26日、三島 | |
小原雄治: 線虫を用いた発生生物学にゲノムサイエンスが与えたインパクト。
第32回日本発生生物学会ワークショップ招待講演、1999年5月29日、神戸 | |
小原雄治: 線虫初期胚における創始細胞、特に生殖系列の運命決定の遺伝子機構。
第22回東北大学加齢医学研究所シンポジウム、1999年6月18-19日、仙台 | |
| 小原雄治: これからのゲノム研究。微生物ゲノムワークショップ、1999年11月25日、瀬戸。 | |
| 小原雄治: cDNA解析を軸とする線虫ゲノム研究の総合戦略。第16回資源生物科学シンポジウム、1999年12月17日、倉敷。 | |
| 小原雄治: ゲノム発生学ー発生のコンピュータモデル化に向けて。公開シンポジウム「プロテオーム時代の計算情報生物学」2000年1月18日、東京。 | |
| 小原雄治: ゲノム生物学-生命システム解明のための総合研究戦略。日本学術会議公開講演会、”「ゲノム科学」の課題と統合的研究推進のための共同体制を考える”、2000年2月4日、東京。 | |
| 小原雄治: 線虫ゲノムの遺伝子発現パターン解析。科研費特定領域A「多元的情報伝達」公開シンポジウム、2000年2月7日、東京。 | |
| 小原雄治: cDNA解析に基づく線虫ゲノムの読み方。科研費特定領域研究C合同公開シンポジウム。2000年7月19日、東京。 | |
| 小原雄治: 「遺伝子はいつ、どの細胞で、どの程度発現するか - 線虫全遺伝子発現パターンの包括的コンピュータ解析」第71回日本動物学会大会シンポジウム、2000年9月22日、東京。 | |
| 小原雄治: 「比較ゲノム発現学をめざして: 線虫発生の遺伝子発現マップ」日本進化学会第2回大会シンポジウム、2000年10月9日、東京。 | |
| 小原雄治: ゲノムから見た生命: 第7回国際生命倫理福井セミナー、2000年11月2日、福井。 | |
| 小原雄治: 「これからのゲノム解析と生命科学技術」第5回関西科学技術セミナー分科会、2000年11月15日、奈良。 | |
| 小原雄治: これからのゲノム科学 Evo, Devoの愉しみ、特定領域(B)「メダカに学ぶ脊椎動物ゲノム再編の分子基盤」公開シンポジウム、2001年1月23日、東京。 | |
| 小原雄治: ゲノムから個体へ-機能ゲノム科学の進展、特定領域A「ゲノムサイエンス」公開シンポジウム、2001年1月26日、東京。 | |
| 小原雄治: 生物進化多様性とゲノム科学の総合、総合研究大学院大学国際シンポジウム一般講演会「21世紀の総合ゲノム科学」、2001年3月17日、横浜。 | |
| 小原雄治: 生命システムの理解とin silico再現をめざして。第28回知能システムシンポジウム、特別講演。2001年3月28日、東京。 | |
| 小原雄治: ヒトゲノム理解に向けて-線虫ゲノムの網羅的発現機能解析。第90回日本病理学会春期総会シンポジウム「疾患モデル研究の戦術·戦略-ポストゲノム時代への視座」2001年4月5日、東京。 | |
| 小原雄治: 「線虫の網羅的な遺伝子発現機能解析」--発生のコンピュータ上再現を目指して--。遺伝研·理研GSC合同公開シンポジウム、2001年6月7日、東京。 | |
| 小原雄治: 遺伝子数のパラドックスーゲノムは生命をどのように規定するのか?総合研究大学院大学講演会、2001年6月9日、東京。 | |
| 2) ポスター発表 (国内60件、海外16件) | |
| Kohara, Y., Motohashi, T., Tabara, H., Sugimoto, A., Watanabe, H., Sano, M., Miyata, A.: Expression map of the C.elegans genome. HGM’96, Heidelberg, Germany, March (1996). | |
| Hiroaki Tabara, Russell Hill, Craig Mello, Tomoko Motohashi, James Priss, Yuji Kohara: A maternal gene, Pos-1, encodes a cytoplasmic Zinc-finger protein and is essential for the fate determination of germline in C.elegans 11th International C.elegans Meeting, May28∼June3 1997, Madison,WI, USA. | |
| Tomoko Motohashi, Hiroaki Tabara, and Yuji Kohara: In situ Hybridization on Larvae and Adults of C.elegans 11th International C.elegans Meeting, May28∼June3 1997, Madison,WI, USA. | |
| Therry-Mieg. J, Thierry-Mieg. D, Shin-i. T, and Kohara. Y,: Expressed genes in C.elegans. 12th International C. elegans Meeting June2-6,1999, Madison, WI, USA | |
| Bertrandy, S., Lefebvre, S., Kohara, Y., Munnich, A. and Thierry-Mieg, D.: RNA interference of SMN, the gene causing Spinal Muscular Atrophy in humans, leads to embryonic lethality and to germ cell apoptosis in the nematode Caenorhabditis elegans. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Hirono, K., Onami, S. and Kohara, Y.: Systematic RNAi experiments with maternal genes. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Ito, M., Motohashi, T., Minakuchi, Y. and Kohara, Y.: Toward four-dimensional database of gene expression in C.elegans. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Maeda, I., Kohara, Y., Yamamoto, M. and Sugimoto, A.: RNAi screening with a non-redundant cDNA set. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Motohashi, T., Ohba, T., Sugiura, I., Obara, M., Kitayama, S., Suzuki, T., Shin-i, T. and Kohara, Y.: Systematic analysis of mRNA distribution by whole mount in situ hybridization. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Onami, S., Nagaoka, T. and Kohara, Y.: Protein expression pattern analysis of maternal mRNAs. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Shin-i, T. and Kohara, Y.: NEXTDB: the nametode expression pattern map database. 12th International C.elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Yoshida, S., Mochii, M., Morita, K., Shimizu, M., Kohara, Y. and Ueno, N.: Isolation and characterization of a gene acting downstream of sma-2 signaling. 12th International C. elegans Meeting, June 2-6, 1999, Madison, WI, USA. | |
| Shin-I, T. and Kohara Y.: NEXTDB: The Expression Pattern Map Dtabase for C.elegans. 10th Workshop on Genome Informatics, Dec. 14-15, 1999, Tokyo. | |
| Atsushi KAJITA, Masayuki YAMAMURA, Yuji KOHARA: Computer Simulation of Early Cleavage of C. elegans Embryo. 13th International C.elegans Meeting, June 22-26 (2001), Los Angeles, USA | |
| Keiko Hirono, Kyoko Nakata, Yumiko Ueta, Mina Iwata, Masahiro Ito and Yuji: Systematic RNAi analysis of maternal genes reveals the function of proteasome in oocyte maturation and fertilization. 13th International C.elegans Meeting, June 22-26 (2001), Los Angeles, USA | |
| Tadasu Shin-i and Yuji Kohara: NEXTDB: The nematode expression pattern database. 13th International C. elegans Meeting, June 22-26 (2001), Los Angeles, USA | |
| (3)新聞報道等 | |
| 日経産業新聞1998年10月14日 | |
| 科学新聞1997年5月30日 | |
| 読売新聞2000年12月25日夕刊 | |
| 朝日新聞2000年8月2日夕刊 | |
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